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Anti-AHR Antibody
Catalog #: 144-01451
AHR Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35869 |
| Gene Id | 196 |
| Gene Symbols | AHR |
| Protein Name / Synonyms | AHR, bHLHe76, aryl hydrocarbon receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 1.59 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
96kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR (NP_001612.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AHR antibody (144-01451) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AHR antibody (144-01451) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.