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Anti-AHSP Antibody
Catalog #: 144-06465
AHSP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NZD4 |
| Gene Id | 51327 |
| Gene Symbols | AHSP |
| Protein Name / Synonyms | AHSP, EDRF, ERAF, alpha-hemoglobin-stabilizing protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a molecular chaperone which binds specifically to free alpha-globin and is involved in hemoglobin assembly. The encoded protein binds to monomeric alpha-globin until it has been transferred to beta-globin to form a heterodimer, which in turn binds to another heterodimer to form the stable tetrameric hemoglobin. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human AHSP (NP_057717.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AHSP antibody (144-06465) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat testis using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AHSP antibody (144-06465) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.