Anti-AIFM1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O95831
Gene Id	9131
Gene Symbols	AIFM1
Protein Name / Synonyms	AIFM1, AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, apoptosis-inducing factor 1, mitochondrial
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

26kDa/28kDa/35kDa/66kDa

Observed MW

67kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AIFM1 antibody (144-02568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Western blot analysis of extracts from normal (control) and AIFM1 knockout (KO) 293T cells, using AIFM1 antibody (144-02568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (144-02568) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded human endometrium using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded mouse esophagus using AIFM1 Antibody (144-02568) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded rat lung using AIFM1 antibody (144-02568) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Immunohistochemistry of paraffin-embedded rat liver using AIFM1 antibody (144-02568) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (144-02568)

Expiration:

12 months from the date of shipment when stored properly.

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