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Anti-AIMP1 Antibody
Catalog #: 144-05301
AIMP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12904 |
| Gene Id | 9255 |
| Gene Symbols | AIMP1 |
| Protein Name / Synonyms | AIMP1, EMAP2, EMAPII, HLD3, SCYE1, p43, aminoacyl tRNA synthase complex-interacting multifunctional protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
34kDa/37kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human AIMP1 (NP_001135888.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AIMP1 Antibody (144-05301) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat spleen using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AIMP1 antibody (144-05301) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AIMP1 antibody (144-05301). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.