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Anti-AIRE Antibody
Catalog #: 144-01767
AIRE Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43918 |
| Gene Id | 326 |
| Gene Symbols | AIRE |
| Protein Name / Synonyms | AIRE, AIRE1, APECED, APS1, APSI, PGA1, autoimmune regulator |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
25kDa/35kDa/36kDa/57kDa
Observed MW
55kDa
Immunogen
A synthetic peptide of human AIRE
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AIRE antibody (144-01767) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using AIRE antibody (144-01767). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.