Anti-AK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P00568
Gene Id	203
Gene Symbols	AK1
Protein Name / Synonyms	AK1, HTL-S-58j, adenylate kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

21kDa

Observed MW

22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AK1 antibody (144-01218) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - AK1 Polyclonal Antibody (144-01218)

Immunohistochemistry of paraffin-embedded human esophageal cancer using AK1 antibody (144-01218) at dilution of 1:100 (40x lens).
Immunohistochemistry - AK1 Polyclonal Antibody (144-01218)

Immunohistochemistry of paraffin-embedded mouse heart using AK1 antibody (144-01218) at dilution of 1:100 (40x lens).
Immunohistochemistry - AK1 Polyclonal Antibody (144-01218)

Immunofluorescence analysis of HeLa cells using AK1 antibody (144-01218). Blue: DAPI for nuclear staining.
Immunofluorescence - AK1 Polyclonal Antibody (144-01218)

Expiration:

12 months from the date of shipment when stored properly.

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