AK2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P54819 |
| Gene Id | 204 |
| Gene Symbols | AK2 |
| Protein Name / Synonyms | AK2, ADK2, adenylate kinase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
14kDa/21kDa/22kDa/24kDa/25kDa/26kDa
Observed MW
32kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AK2 antibody (144-06519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] AK2 Polyclonal Antibody (144-06519)](https://cdn.sanity.io/images/g51potcz/production/ac26bfcb58cb81e4313dbfe33d994815a50320ef-294x350.jpg)
Western blot analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody (144-06519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] AK2 Polyclonal Antibody (144-06519)](https://cdn.sanity.io/images/g51potcz/production/ec2d4fb1f8bc84cd9a3c16c85fde98d2ac46d67a-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using AK2 antibody (144-06519) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (144-06519)](https://www.raybiotech.com/files/images/Antibodies/144/144-06519/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.