Anti-AKAP8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O43823
Gene Id	10270
Gene Symbols	AKAP8
Protein Name / Synonyms	AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95, A-kinase anchor protein 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200

Format

Calculated MW

76kDa

Observed MW

110kDa

Immunogen

A synthetic peptide of human AKAP8

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AKAP8 antibody (144-03049) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - AKAP8 Polyclonal Antibody (144-03049)