3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-AKR1A1 Antibody
Catalog #: 144-60399
AKR1A1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P14550 |
| Gene Id | 10327 |
| Gene Symbols | AKR1A1 |
| Protein Name / Synonyms | AKR1A1, ALDR1, ALR, ARM, DD3, HEL-S-6, alcohol dehydrogenase [NADP(+)] |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
36kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AKR1A1 antibody (144-60399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using AKR1A1 antibody (144-60399) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AKR1A1 antibody (144-60399) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (144-60399).
Expiration:
12 months from the date of shipment when stored properly.