Anti-AKR1B10 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	57016
Protein Name / Synonyms	AKR1B10
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

AKR1B11 antibody, AKR1B12 antibody, ALDRLn antibody, ARL-1 antibody, ARL1 antibody, HIS antibody, HSI antibody, MGC14103 antibody, AKR1B10 antibody, aldo-keto reductase family 1 member B10 antibody, hARP antibody, small intestine reductase antibody, aldose reductase-like peptide antibody, SI reductase antibody, aldose reductase-like 1 antibody, aldo-keto reductase family 1, member B11 (aldose reductase-like) antibody, ARP antibody, aldose reductase-related protein antibody, aldo-keto reductase family 1, member B10 (aldose reductase) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

36 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 316 of AKR1B10 (Uniprot ID# O60218)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 µg mouse colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000

AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 µg rat colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(107-10851) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(107-10851) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: MOLT4
B: Raji
10% SDS PAGE
107-10851 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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