AKT1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P31749 |
| Gene Id | 207 |
| Gene Symbols | AKT1 |
| Protein Name / Synonyms | AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT serine/threonine kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Apoptosis, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
48kDa/55kDa
Observed MW
60kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human AKT1 (NP_005154.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AKT1 antibody (144-011016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat liver using AKT1 antibody (144-011016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using AKT1 antibody (144-011016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AKT1 antibody (144-011016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using AKT1 antibody (144-011016) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AKT1 antibody (144-011016).
Expiration:
12 months from the date of shipment when stored properly.
