ALDH1A1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P00352 |
| Gene Id | 216 |
| Gene Symbols | ALDH1A1 |
| Protein Name / Synonyms | ALDH1A1, ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, retinal dehydrogenase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
54kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (144-01802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.