3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-ALKBH8 Antibody
Catalog #: 144-07142
ALKBH8 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96BT7 |
| Gene Id | 91801 |
| Gene Symbols | ALKBH8 |
| Protein Name / Synonyms | ALKBH8, ABH8, TRM9, TRMT9, alkB homolog 8, tRNA methyltransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:100
IHC 1:100 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
25kDa/27kDa/75kDa
Observed MW
75kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ALKBH8 antibody (144-07142) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using ALKBH8 antibody (144-07142) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ALKBH8 antibody (144-07142) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using ALKBH8 antibody (144-07142). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.