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Anti-alpha-Actin (12-30aa) Antibody
Catalog #: 130-11005-200
Product Description
Specifications
| Size | 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P68133 |
| Gene Id | 58 |
| Gene Symbols | ACTA1 ACTA |
| Protein Name / Synonyms | alpha-Actin (12-30aa) |
| Clonality | Monoclonal |
| Clone | 1E4-H6-E7 |
| Immunogen | Immunogen was a recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Indirect Fluorescent Antibody (IFA) Analysis
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: cytoplasm, cytoskeleton, cytosol
Storage/Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.