RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-alpha-Actin (361-376aa) Antibody

Catalog #: 130-10921-1000

Product Description

Specifications

Size1000 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
Accession Number
P68133
Gene Id
58
Gene Symbols
ACTA1 ACTA
Protein Name / Synonyms
alpha-Actin (361-376aa)
ClonalityPolyclonal
Immunogen
This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen alpha-Actin (361-376aa):KQEYDEAGPSIVHRKC. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography.
Immunogen Sequence
KQEYDEAGPSIVHRKC
Recommended ApplicationsELISA
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Research AreaCytoskeletal Reorganization
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Western Blot:
Western blot image

130-10921 (pAb): 1:2000
10 % SDS-PAGE
Goat anti-rabbit IgG-HRP: 1:5000

Lane: M: protein marker 5 µ

  1. Hela cell cytoplasm fraction 10 µl
  2. 293T cell cytoplasm fraction 10 µl
  3. A549 cell cytoplasm fraction 10 µl

Storage

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.