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Anti-alpha Actinin 4 (internal) Antibody
Catalog #: 107-11047
alpha Actinin 4 antibody [N2C1], Internal, 1.03 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 81 |
| Protein Name / Synonyms | alpha Actinin 4, Internal |
| Concentration (lot specific) | 1.03 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
ACTININ-4 antibody, DKFZp686K23158 antibody, FSGS antibody, FSGS1 antibody, ACTN4 antibody, focal segmental glomerulosclerosis 1 antibody, F-actin cross-linking protein antibody, non-muscle alpha-actinin 4 antibody, alpha-actinin-4 antibody, actinin alpha4 isoform antibody, "actinin, alpha 4 antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
105 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 206 and 542 of alpha Actinin 4 (Uniprot ID# O43707)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
107-11047 diluted at 1:1000
A: NIH-3T3
7.5% SDS PAGE
107-11047 diluted at 1:1000
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11047 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-11047 diluted at 1:1000
alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [N2C1], Internal (107-11047) dilution: 1:3000
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [N2C1], Internal (107-11047) dilution: 1:3000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(107-11047) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(107-11047) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: Hep G2
7.5% SDS PAGE
107-11047 diluted at 1:1000
A: A431
B: Hep G2
7.5% SDS PAGE
107-11047 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.