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Anti-Alpha Smooth Muscle Actin Antibody, Sodium Azide Free
Catalog #: 188-10663-SAF
Mouse anti-Human/Rat Alpha Smooth Muscle Actin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Mouse |
| Accession Number | P62736 |
| Gene Id | 59 |
| Gene Symbols | ACTA2,ACTSA,ACTVS,GIG46 |
| Protein Name / Synonyms | Actin, aortic smooth muscle (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the Alpha Smooth Muscle Actin antibody. |
| Isotype | Kappa, IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Storage
Store the Alpha Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.