Anti-ALPL (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P05186
Gene Id	249
Gene Symbols	ALPL
Protein Name / Synonyms	ALPL (Center)
Clonality	Polyclonal
Preparation	This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the Central region of human ALPL.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

Specificity

Other Names

ALPL; Alkaline phosphatase, tissue-nonspecific isozyme; Alkaline phosphatase liver/bone/kidney isozyme

NCBI Accession #

NP_000469.3;NP_001120973.2;NP_001170991.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

57305

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50; IHC: 1:10-50

	Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) (Cat# 102-12633). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.
	ALPL Antibody (Center) (Cat# 102-12633) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).
	Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat# 102-12633) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	ALPL Antibody (Center) (Cat# 102-12633) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	ALPL Antibody (Center) (Cat# 102-12633)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.