Anti-AMACR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UHK6
Gene Id	23600
Gene Symbols	AMACR
Protein Name / Synonyms	AMACR, AMACRD, CBAS4, P504S, RACE, RM, alpha-methylacyl-CoA racemase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

22kDa/25kDa/42kDa/43kDa

Observed MW

43kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse liver, using AMACR antibody (144-01130) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - AMACR antibody (144-01130)

Expiration:

12 months from the date of shipment when stored properly.

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