Anti-AMPK alpha2 (PRKAA2) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P54646
Gene Id	5563
Gene Symbols	PRKAA2
Protein Name / Synonyms	AMPK alpha2 (PRKAA2) (C-term)
Clonality	Polyclonal
Preparation	This AMPK alpha2 (PRKAA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-483 amino acids from the C-terminal region of human AMPK alpha2 (PRKAA2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	AMPK Signaling, Autophagy, Energy Metabolism
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

NCBI Accession #

NP_006243.2

Other Accession #

Q09137; Q8BRK8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

62320

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of PRKAA2 (arrow) using rabbit polyclonal PRKAA2 Antibody (C-term) (RB11657). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody (C-term)(Cat# 102-17331), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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