AMY2A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04746 |
| Gene Id | 279 |
| Gene Symbols | AMY2A |
| Protein Name / Synonyms | AMY2A, AMY2, PA, pancreatic alpha-amylase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
30kDa/57kDa
Observed MW
58kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AMY2A antibody (144-08045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using AMY2A antibody (144-08045) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using AMY2A antibody (144-08045) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.