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Anti-AMY2B (N-term) Antibody

Catalog #: 102-10761

Rabbit Anti-Human AMY2B (N-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P19961
Gene Id
280
Gene Symbols
AMY2B
Protein Name / Synonyms
AMY2B (N-term)
ClonalityPolyclonal
PreparationThis AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

Specificity

Other Names
AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
NCBI Accession #
NP_066188.1
Other Accession #
P04746; P04745; NP_066188.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
57710
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
AMY2B Antibody (N-term) (Cat# 102-10761) western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).
AMY2B antibody (N-term) (Cat# 102-10761) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
AMY2B Antibody (N-term) (Cat# 102-10761) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AMY2B Antibody (N-term) (Cat# 102-10761) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.