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Anti-Amylin Antibody
Catalog #: RB-08-0028
Rabbit Anti-Human Amylin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_000406 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Amylin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CKCNTATCATQRLANF |
| Recommended Applications | ELISA (recommended work dilution= 5-10 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 ?g/ml. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Amylin is colocalized with somatostatin in endocrine cells of the gastric fundus.
Specificity
The rabbit anti-human Amylin antibody detects specifically both synthetic peptide and cellular protein from 3T3-L1 cell line. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- Dacquin, R., Davey, R.A., Laplace, C., Levasseur, R., Morris, H.A., Goldring, S.R., Gebre-Medhin, S., Galson, D.L., Zajac, J.D., Karsenty, G. Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo. J. Cell Biol. (2004)
- Janson, J., Soeller, W.C., Roche, P.C., Nelson, R.T., Torchia, A.J., Kreutter, D.K., Butler, P.C. Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide. Proc. Natl. Acad. Sci. U.S.A. (1996)
Expiration:
12 months from the date of shipment when stored properly.