Anti-ANGPT2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O15123
Gene Id	285
Gene Symbols	ANGPT2
Protein Name / Synonyms	ANGPT2, AGPT2, ANG2, angiopoietin-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

50kDa/56kDa

Observed MW

75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-496 of human ANGPT2 (NP_001138.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ANGPT2 antibody (144-00698) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - ANGPT2 Polyclonal Antibody (144-00698)

Immunofluorescence analysis of HeLa cells using ANGPT2 antibody (144-00698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - ANGPT2 Polyclonal Antibody (144-00698)

Expiration:

12 months from the date of shipment when stored properly.

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