Anti-ANGPTL4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9BY76
Gene Id	51129
Gene Symbols	ANGPTL4
Protein Name / Synonyms	ANGPTL4, ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158, angiopoietin like 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IF
Research Area	Angiogenesis, Vascular Biology
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

26kDa/40kDa/45kDa

Observed MW

44kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-406 of human ANGPTL4 (NP_647475.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ANGPTL4 antibody (144-02011) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - ANGPTL4 Polyclonal Antibody (144-02011)

Immunofluorescence analysis of A-549 cells using ANGPTL4 antibody (144-02011). Blue: DAPI for nuclear staining.
Immunofluorescence - ANGPTL4 Polyclonal Antibody (144-02011)

Expiration:

12 months from the date of shipment when stored properly.

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