Anti-ANKHD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	Q8IWZ3
Gene Id	54882
Gene Symbols	ANKHD1
Protein Name / Synonyms	ANKHD1, MASK, MASK1, PP2500, VBARP, ankyrin repeat and KH domain containing 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

59kDa/63kDa/64kDa/269kDa/277kDa

Observed MW

269kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1620-1800 of human ANKHD1 (NP_060217.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ANKHD1 antibody (144-08224) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ANKHD1 Polyclonal Antibody (144-08224)

Expiration:

12 months from the date of shipment when stored properly.

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