Anti-ANXA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04083
Gene Id	301
Gene Symbols	ANXA1
Protein Name / Synonyms	ANXA1, ANX1, LPC1, annexin A1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

38kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ANXA1 antibody (144-01118).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)

Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (144-01118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)

Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (144-01118) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)

Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (144-01118) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)

Immunofluorescence analysis of HeLa cells using ANXA1 antibody (144-01118). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (144-01118)

Expiration:

12 months from the date of shipment when stored properly.

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