Anti-ANXA11 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P50995
Gene Id	311
Gene Symbols	ANXA11
Protein Name / Synonyms	ANXA11, ANX11, CAP50, annexin A11
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

51kDa/54kDa

Observed MW

56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 (NP_001148.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ANXA11 antibody (144-07423) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ANXA11 Polyclonal Antibody (144-07423)

Immunofluorescence analysis of A549 cells using ANXA11 antibody (144-07423). Blue: DAPI for nuclear staining.
Immunofluorescence - ANXA11 Polyclonal Antibody (144-07423)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody (144-07423). Western blot was performed from the immunoprecipitate using ANXA11 antibody (144-07423) at a dilition of 1:1000.
Immunoprecipitation - ANXA11 Polyclonal Antibody (144-07423)

Expiration:

12 months from the date of shipment when stored properly.

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