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Anti-AP2A2 Antibody

Catalog #: 144-05391

AP2A2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O94973
Gene Id
161
Gene Symbols
AP2A2
Protein Name / Synonyms
AP2A2, ADTAB, CLAPA2, HIP-9, HIP9, HYPJ, AP-2 complex subunit alpha-2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
73kDa/103kDa/104kDa
Observed MW
118kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human AP2A2 (NP_036437.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AP2A2 antibody (144-05391) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - AP2A2 antibody (144-05391)

Expiration:
12 months from the date of shipment when stored properly.