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Anti-AP3D1 Antibody
Catalog #: 144-60165
AP3D1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O14617 |
| Gene Id | 8943 |
| Gene Symbols | AP3D1 |
| Protein Name / Synonyms | AP3D1, ADTD, HPS10, hBLVR, AP-3 complex subunit delta-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 0.312 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. Defects in this gene are a cause of a new type of Hermansky-Pudlak syndrome.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
111kDa/114kDa/125kDa/130kDa/136kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human AP3D1 (NP_003929.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AP3D1 antibody (144-60165) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.