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Anti-APE1 (N-term) Antibody
Catalog #: 107-10176
APE1 antibody [N1], N-Terminus, 5.04 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 328 |
| Protein Name / Synonyms | APE1, N-term |
| Concentration (lot specific) | 5.04 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, APEX1 antibody, apurinic-apyrimidinic endonuclease 1 antibody, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endonuclease antibody, redox factor-1 antibody, protein REF-1 antibody, AP endonuclease class I antibody, AP lyase antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 45 of APE1 (Uniprot ID# P27695)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (107-10176) dilution: 1:500.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (107-10176) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1(107-10176) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
107-10176 diluted at 1:3000
A: A549
10% SDS PAGE
107-10176 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.