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Anti-APG7L (D555) Antibody

Catalog #: 102-10983

Rabbit Anti-Human APG7L(D555) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
O95352
Gene Id
10533
Gene Symbols
ATG7
Protein Name / Synonyms
APG7L(D555)
ClonalityPolyclonal
PreparationThis APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from human APG7L.
Concentration (lot specific)
0.410 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

Specificity

Predicted Reactivity
Mouse, Rat, Chicken
Other Names
ATG7; APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein
NCBI Accession #
NP_001129503.2;NP_001138384.1;NP_006386.1
Other Accession #
Q641Y5; Q9D906; Q5ZKY2; NP_006386.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
77960
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
hAPG7L-D555 (PEI 1:100)b (Cat# 102-10983) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).
APG7L Antibody(D555) (Cat# 102-10983)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.
APG7L Antibody (Cat# 102-10983) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.