Anti-APOA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	P02647
Gene Id	335
Gene Symbols	APOA1
Protein Name / Synonyms	APOA1, apo(a), apolipoprotein A1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

30kDa

Observed MW

31kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using APOA1 antibody (144-014211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - APOA1 Polyclonal Antibody (144-014211)

Expiration:

12 months from the date of shipment when stored properly.

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