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Anti-APOC1 Antibody

Catalog #: 144-66860

Rabbit anti-Apolipoprotein Recombinant Monoclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P02654
Gene Id
341
Gene Symbols
APOC1
Protein Name / Synonyms
APOC1, Apo-CI, ApoC-I, apo-CIB, apoC-IB, Apolipoprotein C1
ClonalityMonoclonal
Immunogen
A synthesized peptide derived from human APOC1 (Catalog #: 144-65952).
IsotypeIgG
Purification
Affinity purification
Molecular Weight (kDa)
9 kDa
BuffersPBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH 7.3.
Recommended ApplicationsWB, IHC-P, IF/ICC, ELISA
Research AreaGlycolysis & Lipid Metabolism
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. The encoded protein plays a central role in high density lipoprotein (HDL) and very low density lipoprotein (VLDL) metabolism. This protein has also been shown to inhibit cholesteryl ester transfer protein in plasma. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Typical Data

Western blot analysis of lysates from human plasma, using Anti-APOC1 Antibody (Catalog #: 144-66860) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 180s.


Immunohistochemistry analysis of paraffin-embedded Human placenta using Anti-APOC1 Antibody (Catalog #: 144-66860) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Dilutions

IHC 1:50 - 1:100; IHC-P 1:50 - 1:200; IF/ICC 1:50 - 1:200

Storage/Stability

Store at -20°C. Avoid freeze / thaw cycles.

Expiration

12 months from the date of shipment when stored properly.