RayBiotech

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ISO 13485:2016 cGMP

Anti-APRT Antibody

Catalog #: 144-60530

APRT Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P07741
Gene Id
353
Gene Symbols
APRT
Protein Name / Synonyms
APRT, AMP, APRTD, adenine phosphoribosyltransferase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
14kDa/19kDa
Observed MW
19-22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using APRT antibody (144-60530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - APRT antibody (144-60530)

Immunohistochemistry of paraffin-embedded human prostate using APRT Antibody (144-60530) at dilution of 1:100 (40x lens).
Immunohistochemistry - APRT Antibody (144-60530)

Immunohistochemistry of paraffin-embedded mouse lung using APRT Antibody (144-60530) at dilution of 1:100 (40x lens).
Immunohistochemistry - APRT Antibody (144-60530)

Expiration:
12 months from the date of shipment when stored properly.