Anti-ARF3 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	377
Protein Name / Synonyms	ARF3
Concentration (lot specific)	0.57 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ARF3 antibody, small GTP binding protein antibody, ADP-ribosylation factor 3 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

21 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 15 and 181 of ARF3

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1-100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using ARF3(107-10483) antibody at 1:500 dilution.

ARF3 antibody detects ARF3 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ARF3 antibody (107-10483) dilution: 1:500.

ARF3 antibody detects ARF3 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ARF3 antibody (107-10483) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
ADP-ribosylation factor 3 antibody
107-10483 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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