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Anti-ARFGAP1 Antibody
Catalog #: 144-07118
ARFGAP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8N6T3 |
| Gene Id | 55738 |
| Gene Symbols | ARFGAP1 |
| Protein Name / Synonyms | ARFGAP1, ARF1GAP, HRIHFB2281, ADP-ribosylation factor GTPase-activating protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
31kDa/39kDa/44kDa/45kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (144-07118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using ARFGAP1 antibody (144-07118) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ARFGAP1 antibody (144-07118) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using ARFGAP1 antibody (144-07118). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.