Anti-ARG1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P05089
Gene Id	383
Gene Symbols	ARG1
Protein Name / Synonyms	ARG1, arginase-1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	1.42 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

25kDa/34kDa/35kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human ARG1 (NP_000036.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ARG1 Antibody (144-01847) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - ARG1 Polyclonal Antibody (144-01847)

Expiration:

12 months from the date of shipment when stored properly.

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