Anti-ARHGAP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q07960
Gene Id	392
Gene Symbols	ARHGAP1
Protein Name / Synonyms	ARHGAP1, CDC42GAP, RHOGAP, RHOGAP1, p50rhoGAP, rho GTPase-activating protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

50kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ARHGAP1 antibody (144-03739) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - ARHGAP1 Polyclonal Antibody (144-03739)

Immunofluorescence analysis of HeLa cells using ARHGAP1 antibody (144-03739) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ARHGAP1 Polyclonal Antibody (144-03739)

Expiration:

12 months from the date of shipment when stored properly.