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Anti-ARHGAP5 Antibody
Catalog #: 144-03587
ARHGAP5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13017 |
| Gene Id | 394 |
| Gene Symbols | ARHGAP5 |
| Protein Name / Synonyms | ARHGAP5, GFI2, RhoGAP5, p190-B, p190BRhoGAP, rho GTPase-activating protein 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
26kDa/27kDa/172kDa
Observed MW
185kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (144-03587) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (144-03587) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.