Anti-ARHGDIA Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P52565
Gene Id	396
Gene Symbols	ARHGDIA
Protein Name / Synonyms	ARHGDIA, GDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1, rho GDP-dissociation inhibitor 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

18kDa/23kDa

Observed MW

25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ARHGDIA antibody (144-01214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)

Western blot analysis of extracts of various cells, using ARHGDIA antibody (144-01214) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)

Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (144-01214) at 1:1000 dilution.
Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (144-01214)

Expiration:

12 months from the date of shipment when stored properly.

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