ARID3A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q99856 |
| Gene Id | 1820 |
| Gene Symbols | ARID3A |
| Protein Name / Synonyms | ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1, AT-rich interaction domain 3A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
62kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARID3A antibody (144-07668) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (144-07668) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (144-07668) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (144-07668) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody (144-07668). Western blot was performed from the immunoprecipitate using ARID3A antibody (144-07668) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.