ARL6 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9H0F7 |
| Gene Id | 84100 |
| Gene Symbols | ARL6 |
| Protein Name / Synonyms | ARL6, BBS3, RP55, ADP ribosylation factor like GTPase 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described (PMID: 20333246).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
21kDa
Observed MW
21kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human ARL6 (NP_115522.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ARL6 antibody (144-08269) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using ARL6 antibody (144-08269) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ARL6 antibody (144-08269) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using ARL6 antibody (144-08269) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ARL6 antibody (144-08269) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.