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Anti-ARNT2 (Center) Antibody
Catalog #: 102-13038
Rabbit Anti-Human ARNT2 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9HBZ2 |
| Gene Id | 9915 |
| Gene Symbols | ARNT2 |
| Protein Name / Synonyms | ARNT2 (Center) |
| Clonality | Polyclonal |
| Preparation | This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-278 amino acids from the Central region of human ARNT2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting addition roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ARNT2; BHLHE1; KIAA0307; Aryl hydrocarbon receptor nuclear translocator 2; Class E basic helix-loop-helix protein 1
NCBI Accession #
NP_055677.3
Other Accession #
Q78E60; Q61324; NP_055677.3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
78691
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | ARNT2 Antibody (Center) (Cat# 102-13038) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow). |
 | Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center)(Cat# 102-13038). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.