Anti-ASCC1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8N9N2
Gene Id	51008
Gene Symbols	ASCC1
Protein Name / Synonyms	ASCC1, ASC1p50, CGI-18, SMABF2, p50, activating signal cointegrator 1 complex subunit 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in this gene are associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

41kDa/45kDa

Observed MW

46kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ASCC1 (NP_001185727.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of rat pancreas, using ASCC1 antibody (144-61231) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - ASCC1 Polyclonal Antibody (144-61231)

Expiration:

12 months from the date of shipment when stored properly.

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