Anti-ASNA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43681
Gene Id	439
Gene Symbols	ASNA1
Protein Name / Synonyms	ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, TRC40, hASNA-I, ATPase ASNA1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion.

Dilutions

WB 1:200 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

38kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ASNA1 (NP_004308.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ASNA1 antibody (144-03746) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ASNA1 antibody (144-03746)

Immunohistochemistry of paraffin-embedded human stomach using ASNA1 antibody (144-03746) at dilution of 1:100 (40x lens).
Immunohistochemistry - ASNA1 antibody (144-03746)

Expiration:

12 months from the date of shipment when stored properly.

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