Anti-ASPA Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P45381
Gene Id	443
Gene Symbols	ASPA
Protein Name / Synonyms	ASPA, ACY2, ASP, aspartoacylase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

35kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human ASPA (NP_001121557.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ASPA Antibody (144-07271) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - ASPA Antibody (144-07271)

Immunofluorescence analysis of MCF-7 cells using ASPA antibody (144-07271). Blue: DAPI for nuclear staining.
Immunofluorescence - ASPA antibody (144-07271)

Expiration:

12 months from the date of shipment when stored properly.

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