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Anti-ASPSCR1 Antibody
Catalog #: 144-07481
ASPSCR1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9BZE9 |
| Gene Id | 79058 |
| Gene Symbols | ASPSCR1 |
| Protein Name / Synonyms | ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG, UBXD9, UBXN9, tether containing UBX domain for GLUT4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
37kDa/54kDa/60kDa/69kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (144-07481) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (144-07481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (144-07481). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (144-07481) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.