Anti-ATAD3B Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q5T9A4
Gene Id	83858
Gene Symbols	ATAD3B
Protein Name / Synonyms	ATAD3B, AAA-TOB3, TOB3, ATPase family, AAA domain containing 3B
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

19kDa/67kDa/72kDa

Observed MW

73kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATAD3B antibody (144-08268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - ATAD3B antibody (144-08268)

Immunohistochemistry of paraffin-embedded mouse heart using ATAD3B antibody (144-08268) at dilution of 1:100 (40x lens).
Immunohistochemistry - ATAD3B antibody (144-08268)

Immunohistochemistry of paraffin-embedded human liver injury using ATAD3B antibody (144-08268) at dilution of 1:100 (40x lens).
Immunohistochemistry - ATAD3B antibody (144-08268)

Expiration:

12 months from the date of shipment when stored properly.

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