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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P15336 |
| Gene Id | 1386 |
| Gene Symbols | ATF2 |
| Protein Name / Synonyms | ATF2, CRE-BP1, CREB-2, CREB2, HB16, TREB7, activating transcription factor 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
13- 15kDa/23-24kDa/35-54kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF2 (NP_001871.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ATF2 antibody (144-02155) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human mammary gland using ATF2 antibody (144-02155) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using ATF2 antibody (144-02155) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid using ATF2 antibody (144-02155) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ATF2 antibody (144-02155) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using ATF2 antibody (144-02155). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.